RNA

Part:BBa_J64975:Design

Designed by: Kristopher Kuchenbecker   Group: Voigt Lab   (2007-03-23)


anti-glnG RNAi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Wanted an RNAi that would overlap the RBS and start codon for glnG. As an additional constraint on the design, also wanted to allow regulation of the cytosolic levels of this RNAi via an additional RNAi. This was accomplished by extending the 3' end with a G/C-rich region.


Source

E. coli K-12

References